Device

Part:BBa_K4785014

Designed by: Junfei Xia   Group: iGEM23_SUSTech-CHINA   (2023-10-11)


pTetR-LasR-luxR-HMGB1-luxR-pslG-luxR-araC

The tetR promoter constitutively enhances expression of transcriptional factor LasR, which can bind to AHL 3OC12HSL. Then, LasR-3OC12HSL activator complex can open luxR promoter, leading to production target proteins(HMGB1 and pslG) and araC. AraC is a regulator of pBAD promoter, which occurs conformational change in combination with arabinose. Only engineered bacteria exposed to Pseudomonas aeruginosa can sense the arabinose signal, induce lysisE7 expression, lyse themselves and release the target protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 410
    Illegal AgeI site found at 607
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1020
    Illegal BsaI.rc site found at 1755
    Illegal BsaI.rc site found at 3081
    Illegal SapI.rc site found at 1177


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Categories
Parameters
None